Triterpenoids from Ziziphus jujuba induce apoptotic cell death in human cancer cells through mitochondrial reactive oxygen species production.
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大棗鼠李科落葉喬本植物棗，大棗含蛋白質，糖類，有機酸，粘液質，維生素C、P，微量鈣、磷、鐵和多種氨基酸等。近年已有研究顯示提高體內單核－吞噬細胞系統的吞噬功能，保護肝臟，增強肌力和增加體重等作用。而這邊根據Shin M 等人的研究，提取大棗的八種三萜類化合物進行了測試，發現大棗的三萜類化合物可以對於人類的非小細胞肺癌細胞、前列謝癌細胞及乳腺癌細胞通過粒線體的活性氧(ROH)誘導試驗中，結果發現其中4種化合物可以降低癌細胞的存活率，3種可以破壞癌細胞的結構，迫使凋零死亡；這些結果已經讓很多人將大棗視為抗癌新食材。
Ziziphus jujuba var. inermis Rehder is an edible fruit-producing species of the Rhamnaceae family. In the present study, we isolated eight triterpenoids (1-8) from the fruits of Z. jujuba var. inermis and evaluated their apoptotic cell-death-inducing activities in human cancer cell lines (A549, PC-3, and MDA-MB-231). The structures of compounds 1-8 were determined by spectroscopic methods. Among these, four isomers of coumaroyl alphitolic acid showed potent cytotoxic activities on these cancer cells: 3-O-cis-p-coumaroyl alphitolic acid (3), 3-O-trans-p-coumaroyl alphitolic acid (4), 2-O-trans-p-coumaroyl alphitolic acid (5), and 2-O-cis-p-coumaroyl alphitolic acid (6). Moreover, compounds 3-6 induced apoptotic cell death in a concentration-dependent manner. We further investigated the apoptosis-inducing effects of compound 4 in PC-3 cells which triggered the cleavage of procaspase-3, procaspase-7, procaspase-8, bid, and PARP. Compound 4 increased both the mitochondrial reactive oxygen species (ROS) production and the phosphorylation of p38 MAPK (mitogen-activated protein kinase), but decreased the mitochondrial membrane potential. Pretreatment with Mito-TEMPO (a specific mitochondrial-targeted antioxidant) or a specific p38 inhibitor (SB203580) attenuated apoptotic cell death triggered by compound 4 which suggests that compound 4 may induce apoptotic cell death in these cancer cells by increasing the mitochondrial ROS production as well as the subsequent p38 MAPK activation. The study findings provide a rational base to use Ziziphus extracts for cancer treatments in traditional oriental medicine.—文獻摘要
Food Funct. 2018 Jul 17;9(7):3895-3905. doi: 10.1039/c8fo00526e.—文獻出處